Taipei Medical University Hospital

6 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

Chen-Jei Tai^1,2, Chia-Lin Li³, Cheng-Jeng Tai^4,5, Chien-Kai Wang^2,4, Liang-Tzung Lin^3,6

¹Department of Chinese Medicine, Taipei Medical University Hospital, ²Department of Obstetrics and Gynecology, School of Medicine, College of Medicine, Taipei Medical University, ³Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, ⁴Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medical University Hospital, ⁵Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, ⁶Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University

Here, we present a protocol that examines specific steps of the viral entry to identify and evaluate novel antiviral agents.

Medicine

Treatment of Liver Metastases Using an Internal Target Volume Method for Stereotactic Body Radiotherapy

Wei-Jun Wang^1,2, Jeng-Fong Chiou^1,2,3, Yaoru Huang^1,2,4

¹Department of Radiation Oncology, Taipei Medical University Hospital, ²Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, ³Taipei Cancer Center, Taipei Medical University, ⁴Graduate Institute of Biomedical Materials & Tissue Engineering, College of Biomedical Engineering, Taipei Medical University

Stereotactic body radiotherapy (SBRT) requires rigorous accuracy and precision for delivering high radiation doses per fraction to small treatment volumes to improve tumor control and simultaneously reduce toxicity. Herein, we present a noninvasive and clinically convenient respiratory motion management protocol for SBRT for liver metastases.

Medicine

Two-vessel Occlusion Mouse Model of Cerebral Ischemia-reperfusion

Cheng-Yu Chen^*1,2,3,4,5, Ray-Jade Chen^*6,7, Gilbert Aaron Lee⁵

A mouse model of cerebral ischemia-reperfusion is established to investigate the pathophysiology of stroke. We distally ligate the right middle cerebral artery and right common carotid artery and restore blood flow after 10 or 40 min of ischemia.

Medicine

Tracking Superparamagnetic Iron Oxide-labeled Mesenchymal Stem Cells using MRI after Intranasal Delivery in a Traumatic Brain Injury Murine Model

Rami Ahmad Shahror^1,2,3, Chung-Che Wu^2,3,4,5, Yung-Hsiao Chiang^1,2,3,4,5, Kai-Yun Chen^1,2,3

¹Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University and National Health Research Institutes, ²Center for Neurotrauma and Neuroregeneration, Taipei Medical University, ³TMU Neuroscience Research Center, Taipei Medical University, ⁴Department of Neurosurgery, Taipei Medical University Hospital, ⁵Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University

Presented here is a protocol for non-invasive mesenchymal stem cell (MSC) delivery and tracking in a mouse model of traumatic brain injury. Superparamagnetic iron oxide nanoparticles are employed as a magnetic resonance imaging (MRI) probe for MSC labeling and non-invasive in vivo tracking following intranasal delivery using real-time MRI.

Medicine

Real-Time Magnetic Resonance Guided Focused Ultrasound for Painful Bone Metastases

Wei-Jun Wang¹, Hsin-Lun Lee^1,2, Shiu-Chen Jeng^1,3, Jeng-Fong Chiou^1,4,5, Yaoru Huang^1,6

¹Department of Radiation Oncology, Taipei Medical University Hospital, ²The Ph.D. Program for Translational Medicine, College of Medical Science and Technology, Taipei Medical University and Academia Sinica, Taipei Medical University, ³School of Dentistry, College of Oral Medicine, Taipei Medical University, ⁴Department of Radiology, School of Medicine, College of Medicine, Taipei Medical University, ⁵Taipei Cancer Center, Taipei Medical University, ⁶Graduate Institute of Biomedical Materials & Tissue Engineering, College of Biomedical Engineering, Taipei Medical University

Magnetic resonance could offer real-time monitoring of the position and temperature of focused ultrasound in thermal ablation for painful bone metastases, regardless of cancer type or previous local treatments. Our innovative method of quality assurance could facilitate the application of this effective and safe treatment.

Medicine

Intratracheal Instillation of Stem Cells in Term Neonatal Rats

Chung-Ming Chen^1,2, Yue-Jun Chen¹, Zheng-Hao Huang¹

¹Department of Pediatrics, School of Medicine, College of Medicine, Taipei Medical University, ²Department of Pediatrics, Taipei Medical University Hospital

Described is a protocol for performing intratracheal transplantation of mesenchymal stromal cells (MSCs) through intratracheal injection in term neonatal rats. This technique is a clinically viable option for delivery of stem cells and drugs into neonatal rat lungs to evaluate their efficacy.