Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells (MSCs)Pei-Ju Hsu 1, Ko-Jiunn Liu 2, Ying-Yin Chao 1, Huey-Kang Sytwu 3, B. Linju Yen 1
1Regenerative Medicine Research Group, Institute of Cellular & System Medicine, National Health Research Institutes (NHRI), 2National Institute of Cancer Research, National Health Research Institutes (NHRI), 3Institute of Microbiology & Immunology, National Defense Medical Center
The immunomodulatory properties of human mesenchymal stem cells (MSC) appear increasingly relevant for clinical application. Using a co-culture system of MSCs and peripheral blood leukocytes pre-stained with the fluorescent dye carboxyfluorescein succinimidyl ester (CFSE), we describe the in vitro assessment of MSC immunomodulation on effector leukocyte proliferation and specific subpopulations.
