The Hebrew University–Hadassah Faculty of Dental Medicine

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts

Doron Cohn Yakubovich¹, Wafa Tawackoli^2,3,4, Dmitriy Sheyn^2,3, Ilan Kallai¹, Xiaoyu Da⁴, Gadi Pelled^1,2,3,4, Dan Gazit^1,2,3,4, Zulma Gazit^1,2,3

¹Skeletal Biotech Laboratory, The Hebrew University–Hadassah Faculty of Dental Medicine, ²Department of Surgery, Cedars-Sinai Medical Center, ³Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, ⁴Biomedical Imaging Research Institute, Cedars-Sinai Medical Center

Implantation of autologous and allogeneic bone grafts constitute accepted approaches to treat major craniofacial bone loss. Yet the effect of graft composition on the interplay among neovascularization, cell differentiation and bone formation is unclear. We present a multimodal imaging protocol aimed to elucidate the angiogenesis-osteogenesis interdependence at the graft proximity.

Bioengineering

Semiautomated Longitudinal Microcomputed Tomography-based Quantitative Structural Analysis of a Nude Rat Osteoporosis-related Vertebral Fracture Model

Galina Shapiro¹, Maxim Bez¹, Wafa Tawackoli^2,3,4, Zulma Gazit^1,2,3,5, Dan Gazit^1,2,3,4,5, Gadi Pelled^1,2,3,4

¹Skeletal Biotech Laboratory, Hebrew University-Hadassah Faculty of Dental Medicine, ²Department of Surgery, Cedars-Sinai Medical Center, ³Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, ⁴Biomedical Imaging Research Institute, Cedars-Sinai Medical Center, ⁵Department of Orthopedics, Cedars-Sinai Medical Center

The goal of this protocol is to generate a nude rat osteoporosis-related vertebral compression fracture model that can be longitudinally evaluated in vivo using a semiautomated microcomputed tomography-based quantitative structural analysis.