CNRS UMR 7225

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Philip O'Herron¹, Zhiming Shen¹, Zhongyang Lu¹, Adrien E. Schramm¹, Manuel Levy¹, Prakash Kara¹

¹Department of Neuroscience, Medical University of South Carolina

A method is described for labeling neurons with fluorescent dyes in predetermined functional micro-domains of the neocortex. First, intrinsic signal optical imaging is used to obtain a functional map. Then two-photon microscopy is used to label and image neurons within a micro-domain of the map.

Neuroscience

Induction of an Isoelectric Brain State to Investigate the Impact of Endogenous Synaptic Activity on Neuronal Excitability In Vivo

Tristan Altwegg-Boussac^1,2,3,4, Séverine Mahon^1,2,3,4, Mario Chavez^1,2,3,4, Stéphane Charpier^1,2,3,4, Adrien E. Schramm^1,2,3,4

¹Inserm U1127, ²CNRS UMR 7225, ³UPMC Univ Paris 06, UMR S 1127, Sorbonne Universités, ⁴Institut du Cerveau et de la Moelle épinière (ICM)

This procedure performs long-lasting in vivo intracellular recordings from single neurons during physiologically relevant cerebral states and after complete abolition of ongoing electrical activities, resulting in an isoelectric brain state. The physiological constants of the animal are carefully monitored during the transition to the artificial comatose condition.