UMR-9197, CNRS/Université Paris Sud

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

In Vivo Functional Brain Imaging Approach Based on Bioluminescent Calcium Indicator GFP-aequorin

Arianna R. Lark^1,2, Toshihiro Kitamoto^2,3, Jean-René Martin¹

¹Equipe: Imagerie Cérébrale Fonctionnelle et Comportements (ICFC), Institut des Neurosciences Paris-Saclay (Nero-PSI), UMR-9197, CNRS/Université Paris Sud, ²Interdisciplinary Program in Neuroscience, Graduate College, University of Iowa, ³Department of Anesthesia, Carver College of Medicine, University of Iowa

Here we present a novel Ca²⁺-imaging approach using a bioluminescent reporter. This approach uses a fused construct GFP-aequorin which binds to Ca²⁺ and emits light, eliminating the need for light excitation. Significantly this method permits long continuous imaging, access to deep brain structures and high temporal resolution.