National Institute of Dental and Craniofacial Research, NIH, DHHS

2 ARTICLES PUBLISHED IN JoVE

Medicine

Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct

Yusuke Kuriki¹, Younan Liu¹, Dengsheng Xia¹, Eva M. Gjerde¹, Saeed Khalili¹, Brennan Mui¹, Changyu Zheng², Simon D. Tran¹

¹Faculty of Dentistry, McGill University , ²National Institutes of Health, Bethesda, MD, USA

A protocol for the cannulation of the mouse submandibular salivary gland via the Wharton's duct is described. For this experiment, the trypan blue solution is used as a dyer to demonstrate how this technique effectively delivers infusions into the targeted gland, and to suggest the reliability of this new approach as a potential clinical drug/cell therapy for the regeneration of salivary glands.

Medicine

Retroductal Submandibular Gland Instillation and Localized Fractionated Irradiation in a Rat Model of Salivary Hypofunction

Renjith Parameswaran Nair¹, Changyu Zheng², Gulshan Sunavala-Dossabhoy¹

¹Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, ²Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, NIH, DHHS

Salivary gland hypofunction, a major adverse effect of head and neck radiotherapy diminishes a patient's quality of life. The demonstration of efficacy of new therapies in animal models is a prerequisite before clinical transition. This protocol describes retroductal administration and local irradiation of rat submandibular glands.