Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various TissuesJulia Barthelmes *1, Nadja Tafferner *2, Jennifer Kurz 2, Natasja de Bruin 2, Michael J. Parnham 2, Gerd Geisslinger 1, Susanne Schiffmann 2
1Institute of Clinical Pharmacology, Goethe University Hospital Frankfurt, 2Project Group for Translational Medicine & Pharmacology, Fraunhofer IME
This manuscript describes the methods for induction and scoring of the experimental autoimmune encephalomyelitis (EAE) model, together with the assessment of immune cell distribution and mRNA cytokine levels in lymph nodes, spleen, blood and spinal cord using flow cytometry and quantitative PCR, respectively, at various disease phases.
