Canadian Institute for Advanced Research

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Foraging Path-length Protocol for Drosophila melanogaster Larvae

Ina Anreiter¹, Oscar E. Vasquez¹, Aaron M. Allen², Marla B. Sokolowski^1,3

¹Department of Ecology and Evolutionary Biology, University of Toronto, ²Department of Cell and Systems Biology, University of Toronto, ³Child and Brain Development Program, Canadian Institute for Advanced Research

We provide a detailed protocol for a Drosophila melanogaster foraging path-length assay. We discuss the preparation and handling of test animals, how to perform the assay and analyze the data.

Immunology and Infection

T4 Bacteriophage and E. coli Interaction in the Murine Intestine: A Prototypical Model for Studying Host-Bacteriophage Dynamics In Vivo

Nicola Pett¹, Michael Hunter¹, Natalia A. Carranza García¹, Jung Hee Seo¹, Samuel R. Collins¹, Forest Rohwer², Lisa C. Osborne¹, Carolina Tropini^1,3,4

¹Department of Microbiology and Immunology, Life Sciences Institute, University of British Columbia, ²Department of Biology, San Diego State University, ³School of Biomedical Engineering, University of British Columbia, ⁴Humans and the Microbiome Program, Canadian Institute for Advanced Research

Bacteriophages (phages), viruses that infect bacteria, are an integral component of the gut microbiome. Though these symbiotic inhabitants drive bacterial fitness and population dynamics, little is understood about how they impact gut homeostasis and disease. This protocol studies isolated T4 phages within a mouse model, adaptable to other phage-bacterial pairs.