National University of Ireland Galway
2 ARTICLES PUBLISHED IN JoVE
Environment
Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
Enrico Tatti 1, Boyd A. McKew 2, Corrine Whitby 2, Cindy J. Smith 1
1Microbiology, School of Natural Sciences, National University of Ireland Galway, 2School of Biological Sciences, University of Essex
A protocol to quantify bacterial 16S rRNA genes and transcripts from coastal sediments via real-time PCR is provided. The methodology includes the co-extraction of DNA and RNA; preparation of DNA-free RNA; and 16S rRNA gene and transcript quantification via RT-q-PCR, including standard curve construction.
Bioengineering
Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery
Raymond Wing Moon Lam 1, Sunny Akogwu Abbah 2, Wang Ming 1, Mathanapriya Naidu 1, Felly Ng 1, Hu Tao 1, James Goh Cho Hong 3,4, Kang Ting 5, Wong Hee Kit 1,4
1Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore, 2Centre for Research in Medical Devices (CÚRAM), National University of Ireland Galway, 3Department of Bioengineering, Faculty of Engineering, National University of Singapore, 4Tissue Engineering Program, National University of Singapore, 5Department of Orthopaedic Surgery and the Orthopaedic Hospital Research Center, University of California, Los Angeles
Self-assembled polyelectrolyte complexes (PEC) fabricated from heparin and protamine were deposited on alginate beads to entrap and regulate the release of osteogenic growth factors. This delivery strategy enables a 20-fold reduction of BMP-2 dose in spinal fusion applications. This article illustrates the benefits and fabrication of PECs.
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