Ingham Institute for Applied Medical Research

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

Murray C. Killingsworth^1,2,3,4, Yuri V. Bobryshev^3,4,5

¹South Western Sydney Clinical School, Faculty of Medicine, University of New South Wales Australia, ²School of Medicine, Western Sydney University, ³Correlative Microscopy Group, Ingham Institute for Applied Medical Research, ⁴Electron Microscopy Laboratory, Department of Anatomical Pathology, Sydney South West Pathology Service, New South Wales Health Pathology, ⁵School of Medical Sciences, Faculty of Medicine, University of New South Wales Australia

A method is described whereby quantum dot (QD) nanoparticles can be used for correlative immunocytochemical studies of epoxy embedded human pathology tissue. We employ commercial antibody fragment conjugated QDs that are visualized by widefield fluorescence light microscopy and transmission electron microscopy.

Cancer Research

An Orthotopic Resectional Mouse Model of Pancreatic Cancer

Tony C. Y. Pang^1,2,4,5, Zhihong Xu^1,2, Alpha Raj Mekapogu^1,2, Srinivasa Pothula^1,2, Therese M. Becker³, David Goldstein¹, Romano C. Pirola^1,2, Jeremy S. Wilson^1,2, Minoti V. Apte^1,2

¹Pancreatic Research Group, South Western Sydney Clinical School, University of New South Wales, ²Ingham Institute for Applied Medical Research, ³Centre for Circulating Tumour Cell Diagnostics and Research, Ingham Institute for Applied Medical Research, ⁴Surgical Innovations Unit, Westmead Hospital, ⁵Westmead Clinical School, University of Sydney

In the clinical context, patients with localized pancreatic cancer will undergo pancreatectomy followed by adjuvant treatment. This protocol reported here aims to establish a safe and effective method of modelling this clinical scenario in nude mice, through orthotopic implantation of pancreatic cancer followed by distal pancreatectomy and splenectomy.