University of Maryland-Baltimore School of Medicine
2 ARTICLES PUBLISHED IN JoVE
Medicine
The Rodent Model of Nonarteritic Anterior Ischemic Optic Neuropathy (rNAION)
Yan Guo 1, Zara Mehrabian 1, Steven L. Bernstein 1,2
1Department of Ophthalmology and Visual Sciences, University of Maryland-Baltimore School of Medicine, 2Anatomy and Neurobiology, University of Maryland-Baltimore School of Medicine
The following report describes how to replicate the rodent model of nonarteritic anterior ischemic optic neuropathy (rNAION), using the appropriate dye, contact lens and laser parameters. We also reveal the appropriate steps for evaluating the rNAION lesion in vivo.
JoVE Core
Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)
Yohei Niikura *1, Lei Fang *2, Risa Kitagawa *3, Peizhao Li 1, Yao Xi 1, Ju You 1, Yan Guo 2, Katsumi Kitagawa 3
1MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center, Nanjing University, 2Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, 3Greehey Children's Cancer Institute, Department of Molecular Medicine, UT Health Science Center San Antonio
CENP-A ubiquitylation is an important requirement for CENP-A deposition at the centromere, inherited through dimerization between cell division, and indispensable to cell viability. Here we describe mass spectrometry analysis to identify ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A) protein.
