Guangdong General Hospital, Guangdong Academy of Medical Sciences
2 ARTICLES PUBLISHED IN JoVE
Medicine
Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms
Manav Korpal 1, Jacob Feala 1, Xiaoling Puyang 1, Jian Zou 1, Alex H. Ramos 2, Jeremy Wu 1, Timm Baumeister 1, Lihua Yu 1, Markus Warmuth 1, Ping Zhu 1
1H3 Biomedicine, 2Massachusetts General Hospital
Drug resistance to targeted therapeutics is widespread and the need to identify mechanisms of resistance--prior to or following clinical onset--is critical for guiding alternative clinical management strategies. Here, we present a protocol to couple derivation of drug-resistant lines in vitro with sequencing to expedite discovery of these mechanisms.
Developmental Biology
Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy
Haochuan Zhang *1,2,3, JunGang Huang *2,3,4, Xin Liu 2,3, Ping Zhu 4, Zhongrong Li 1, Xue Li 2,3
1Department of Pediatric Surgery, The Second Affiliated Hospital & Yuying Children's Hospital, Wenzhou Medical University, 2Departments of Urology, Boston Children's Hospital, 3Department of Surgery, Harvard Medical School, 4Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Sciences
We describe a detailed protocol using high-resolution episcopic microscopy to acquire three-dimensional (3D) images of mouse embryos. This improved protocol utilizes a modified tissue preparation method to enhance penetration of the fluorescent dye, thereby permitting morphometric analysis of both small and large-sized specimens.
