Paul-Ehrlich-Institute

2 ARTICLES PUBLISHED IN JoVE

Medicine

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Gunnar T.R. Hischebeth¹, Sascha Gravius², Johanna K. Buhr², Ernst Molitor¹, Matthias D. Wimmer², Achim Hoerauf¹, Isabelle Bekeredjian-Ding³, Thomas M. Randau²

¹Institute of Medical Microbiology, Immunology and Parasitology, University Hospital Bonn, ²Department of Orthopaedics and Trauma Surgery, University Hospital Bonn, ³Division of EU Cooperation/Microbiology, Paul-Ehrlich-Institute

Differential diagnostics in painful arthroplasty is crucial for treatment success. Joint aspiration is routinely performed preoperatively. Multiplex polymerase chain reaction of the joint aspirate and the sonication fluid, a feasible tool for quick pathogen detection from these samples is described.

Medicine

Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System

Sandra Johnen¹, Nina Harmening^2,3, Corinne Marie^4,5, Daniel Scherman⁴, Zsuzsanna Izsvák⁶, Zoltán Ivics⁷, Peter Walter¹, Gabriele Thumann^2,3

¹Department of Ophthalmology, University Hospital RWTH Aachen, ²Experimental Ophthalmology, University of Geneva, ³Department of Ophthalmology, University Hospitals of Geneva, ⁴Université de Paris, CNRS, INSERM, UTCBS, Unité des technologies Chimiques et Biologiques pour la Santé, ⁵Chimie ParisTech, PSL Research University, ⁶Max Delbrück Center for Molecular Medicine in the Helmholtz Association, ⁷Division of Medical Biotechnology, Paul-Ehrlich-Institute

We have developed a protocol to transfect primary human pigment epithelial cells by electroporation with the gene encoding pigment epithelium-derived factor (PEDF) using the Sleeping Beauty (SB) transposon system. Successful transfection was demonstrated by quantitative polymerase chain reaction (qPCR), immunoblotting, and enzyme-linked immunosorbent assay (ELISA).