Eberhard Karls University of Tübingen
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
Johannes Schwenck 1,2, Florian C. Maier 1, Manfred Kneilling 1,3, Stefan Wiehr 1, Kerstin Fuchs 1
1Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, Eberhard Karls University of Tübingen, 2Department of Nuclear Medicine, Eberhard Karls University of Tübingen, 3Department of Dermatology, Eberhard Karls University of Tübingen
This paper explains the application of fluorescent imaging using an activatable optical imaging probe to visualize the in vivo activity of key matrix metalloproteinases in two different experimental models of inflammation.
Immunology and Infection
Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
Sabrina H. L. Hoffmann 1, Andreas Maurer 1, Dorothea I. Reck 1, Gerald Reischl 1, Bernd J. Pichler 1, Manfred Kneilling 1,2, Christoph M. Griessinger 1
1Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, Eberhard Karls University Tübingen, 2Department of Dermatology, Eberhard Karls University Tübingen
Following the preparation of a 64Cu-modified monoclonal antibody binding to a transgenic murine T cell receptor, T cells are radiolabeled in vivo, analyzed for viability, functionality, labeling stability and apoptosis, and adoptively transferred into mice with an airway delayed-type hypersensitivity reaction for non-invasive imaging by positron emission tomography/computed tomography (PET/CT).
