Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read SequencingJoanna M. Feehan *1,2, Katherine E. Scheibel *1, Salim Bourras 3, William Underwood 4, Beat Keller 3, Shauna C. Somerville 1
1Department of Plant and Microbial Biology, University of California Berkeley, 2John Innes Centre, Norwich Research Park, 3Department of Plant and Microbial Biology, University of Zürich, 4USDA-ARS Sunflower and Plant Biology Research Unit
Described here is a method for the extraction, purification, and quality control of genomic DNA from the obligate biotrophic fungal pathogen, powdery mildew, for use in long-read genome sequencing.
