AOUI Verona

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses

Gabriella Bergamini^*1, Fabio Stellari^*2, Angela Sandri^*3, Maria M. Lleo³, Gaetano Donofrio⁴, Francesca Ruscitti^5,2, Federico Boschi⁶, Andrea Sbarbati⁷, Gino Villetti², Paola Melotti⁸, Claudio Sorio¹

¹Department of Medicine, General Pathology Division, Cystic Fibrosis Translational Research Laboratory "D. Lissandrini", University of Verona, ²Corporate Preclinical R&D, Chiesi Farmaceutici S.p.A., ³Department of Diagnostic and Public Health, Microbiology Division, University of Verona, ⁴Dipartimento di Scienze Medico-Veterinarie, University of Parma, ⁵Department of Biomedical Biotechnological and Translational Sciences, University of Parma, ⁶Department of Computer Science, University of Verona, ⁷Department of Neurological, Biomedical and Movement Sciences, University of Verona, ⁸Cystic Fibrosis Regional Center (CFC), AOUI Verona

The method described here allows for the visualization of IL-8 promoter-dependent inflammation activation in the lungs of mice through non-invasive bioluminescence imaging (BLI). The same animal can be subjected to BLI multiple times for up to two months from the time of delivery of the luciferase reporter construct.

Medicine

Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)

George M. Solomon¹, Inez Bronsveld², Kathryn Hayes³, Michael Wilschanski⁴, Paola Melotti⁵, Steven M. Rowe¹, Isabelle Sermet-Gaudelus^6,7

¹Department of Medicine and the Gregory Fleming James Cystic Fibrosis Center, University of Alabama at Birmingham, ²Department of Pulmonology and Tuberculosis, University Medical Center Utrecht, ³Center for Experimental Medicine, Queens University, Northern Ireland, ⁴Hadassah Hebrew University Medical Center, Jerusalem, ⁵Centro Fibrosi Cistica, Azienda Ospedaliera Universitaria Integrata, ⁶Service de Pneumologie et Allergologie Pédiatriques and Center de Ressources et de Compétence de la Mucoviscidose, Hôpital Necker Enfants Malades, ⁷INSERM U 1151, Institut Necker Enfants Malades

Here, we present a standardized protocol to measure the nasal potential difference (NPD). Cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC) function are evaluated by the change in the voltage across the nasal epithelium after superfusion of solutions that modify ion channel activity, providing an outcome measure.