Université Paris Descartes-Sorbonne Paris Cité

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

Pascale Vonaesch¹, Philippe J. Sansonetti^1,2, Pamela Schnupf³

¹Unité de Pathogénie Microbienne Moléculaire, INSERM U786, Institut Pasteur, ²Microbiologie et Maladies Infectieuses, Collège de France, ³Laboratory of Intestinal Immunity, Institut Imagine-INSERM UMR 1163, Université Paris Descartes-Sorbonne Paris Cité

We describe a method for the qualitative and quantitative analysis of stress granule formation in mammalian cells after the cells are challenged with bacteria and a number of different stresses. This protocol can be applied to investigate the cellular stress granule response in a wide range of host-bacterial interactions.