Macromolecular Complexes

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Light Sheet-based Fluorescence Microscopy of Living or Fixed and Stained Tribolium castaneum Embryos

Frederic Strobl^1,2,3, Selina Klees^1,2,3, Ernst H. K. Stelzer^1,2,3

¹Physical Biology, Buchmann Institute for Molecular Life Sciences (BMLS), ²Cluster of Excellence Frankfurt, Macromolecular Complexes, ³Goethe-Universität Frankfurt am Main – Campus Riedberg

Imaging the morphogenesis of insect embryos with light sheet-based fluorescence microscopy has become state of the art. This protocol outlines and compares three mounting techniques appropriate for Tribolium castaneum embryos, introduces two novel custom-made transgenic lines well suited for live imaging, discusses essential quality controls and indicates current experimental limitations.

Biology

Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

Julia Ratke^*1, Franziska Krämer^*1, Frederic Strobl¹

¹Physical Biology/Physikalische Biologie (IZN, FB15), Buchmann Institute for Molecular Life Sciences (BMLS), Cluster of Excellence Frankfurt, Macromolecular Complexes (CEF - MC), Goethe-Universität

Light sheet-based fluorescence microscopy is the most valuable tool in developmental biology. A major issue in comparative studies is ambient variance. Our protocol describes an experimental framework for simultaneous live imaging of multiple specimens and, therefore, addresses this issue pro-actively.