Complutense University School of Medicine

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N⁶-threonylcarbamoyladenosine TcdA Function

Francisco J. Fernández^1,2,3, Sara Gómez¹, Sergio Navas-Yuste¹, Miguel López-Estepa¹, M. Cristina Vega¹

¹Department of Physical Chemical Biology, Center for Biological Research (CIB-CSIC), Spanish National Research Council (CSIC), ²Department of Immunology, Complutense University School of Medicine, ³Abvance Biotech srl

A protocol is presented for the production of tRNA(UUU) and the analysis of tRNA(UUU) in complex with the enzyme TcdA by agarose gel retardation assays.