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Chinese Academy of Science/Shanghai Jiao Tong University School of Medicine

1 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay
Yuetian Zhang *1, Yi Pan *1, Hiroaki Matsunami 2,3, Hanyi Zhuang 1,4
1Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of National Ministry of Education, Shanghai Jiao Tong University School of Medicine, 2Department of Molecular Genetics and Microbiology, Duke University Medical Center, 3Department of Neurobiology, Duke Institute for Brain Sciences, Duke University Medical Center, 4Institute of Health Science, Chinese Academy of Science/Shanghai Jiao Tong University School of Medicine

Characterizing the function of odorant receptors serves an indispensable part in the deorphanization process. We describe a method to measure the activation of odorant receptors in real time using a cAMP assay.

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