Laboratorio de Biología del Desarrollo

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays

Carmen Bolatto¹, Cristina Parada¹, Victoria Colmenares¹

¹Departamento de Histología y Embriología, Facultad de Medicina, Laboratorio de Biología del Desarrollo

The ability to accurately detect transcripts or proteins in Drosophila tissues is critical for studying their abundance and localization related to the development process. Here is the description of a straightforward procedure to dissect pupal wings. These wings can be used as samples in several techniques (immunohistochemistry, PCR assay, etc.).

Neuroscience

Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions

Carmen Bolatto^*1, Silvia Olivera-Bravo^*2, Sofía Cerri^1,2

¹Developmental Biology Laboratory, Histology and Embryology Department, Faculty of Medicine, Universidad de la República, ²Cell and Molecular Neurobiology Laboratory, Clemente Estable Biology Research Institute (IIBCE), Ministerio de Educación y Cultura

The ability to accurately detect neuromuscular junction components is crucial in evaluating modifications in its architecture because of pathological or developmental processes. Here we present a complete description of a straightforward method to obtain high-quality images of whole-mount neuromuscular junctions that can be used to perform quantitative measurements.