Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA LesionsSoňa Legartová *1, Jana Suchánková *1, Jana Krejčí 1, Alena Kovaříková 1, Eva Bártová 1
1Institute of Biophysics of the Czech Academy of Sciences
Laser microirradiation is a useful tool for studies of DNA repair in living cells. A methodological approach for the use of UVA lasers to induce various DNA lesions is shown. We have optimized a method for local microirradiation that maintains the normal cell cycle; thus, irradiated cells proceed through mitosis.
