Szent István University

3 ARTICLES PUBLISHED IN JoVE

Biology

Cryopreservation of Zebrafish Spermatogonia by Whole Testes Needle Immersed Ultra-Rapid Cooling

Zoran Marinović¹, Jelena Lujić¹, Eszter Kása¹, Zsolt Csenki¹, Béla Urbányi¹, Ákos Horváth¹

¹Department of Aquaculture, Szent István University

The main purpose of this study was to adapt the needle immersed vitrification (NIV) procedure to cryopreserve whole zebrafish testes. Additionally, the repeatability of the method in five different zebrafish strains was tested.

Developmental Biology

Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation

Juan G. Herranz-Jusdado¹, Eszter Kása², Tímea Kollár², Víctor Gallego¹, David S. Peñaranda¹, Christoffer Rozenfeld¹, Luz Pérez¹, Ákos Horváth², Juan F. Asturiano¹

¹Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, ²Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Szent Istvan University

Protocols for European eel maturation and sperm cryopreservation have been improved over the last years. This article describes the best protocol available using human chorionic gonadotropin (hCG) for inducing maturation and methanol as cryoprotectant.

Environment

Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds

Zsolt Csenki¹, Ákos Horváth¹, Illés Bock¹, Edina Garai¹, Flóra Kerekes¹, Erna Vásárhelyi¹, Balázs Kovács¹, Béla Urbányi¹, Ferenc Mueller², Katalin Bakos¹

¹Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences, Szent István University, ²Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham

Present here is a detailed protocol for the use of zebrafish embryos Tg(vtg1: mCherry) for the detection of estrogenic effects. The protocol covers the propagation of the fish and treatment of embryos, and emphasizes the detection, documentation, and the evaluation of fluorescent signals induced by endocrine disrupting compounds (EDC).