United States Army Medical Research Institute of Infectious Diseases (USAMRIID)

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment

Candace D. Blancett¹, Mitchell K. Monninger¹, Chrystal A. Nguessan¹, Kathleen A. Kuehl¹, Cynthia A. Rossi², Scott P. Olschner², Priscilla L. Williams², Steven L. Goodman³, Mei G. Sun¹

¹Pathology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), ²Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), ³Microscopy Innovations LLC

This protocol provides instruction for negative staining virus samples which can easily be used in BSL-2, -3, or -4 laboratories. It includes the use of an innovative processing capsule, which protects the transmission electron microscopy grid and provides the user easier handling in the more turbulent environments within biocontainment.