Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In VivoVeronica Akle 1, Nathalie Agudelo-Dueñas *1,2, Maria A. Molina-Rodriguez *1, Laurel Brianne Kartchner 1,3,4,6, Annette Marie Ruth 1,3,5,6, John M. González 3, Manu Forero-Shelton 2
1Laboratory of Neurosciences and Circadian Rhythms, School of Medicine, Universidad de los Andes, 2Biophysics Group, Department of Physics, Universidad de los Andes, 3Laboratory of Basic Medical Sciences, School of Medicine, Universidad de los Andes, 4Department of Microbiology and Immunology, University of North Carolina, 5Notre Dame Initiative for Global Development, University of Notre Dame, 6USAID Research and Innovation Fellowship program
In this protocol, fluorescently labeled T. cruzi were injected into transparent zebrafish larvae, and parasite motility was observed in vivo using light sheet fluorescence microscopy.
