Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron PrecursorsDavid J. Tischfield 1,2, Stewart A. Anderson 1
1Neuroscience Graduate Group, Perelman School of Medicine, University of Pennsylvania, 2Department of Psychiatry, Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine
This protocol describes a method for generating cortical interneuron progenitors and post-mitotic interneuron precursors from mouse embryonic stem cells using a modified embryoid body-to-monolayer method. These progenitors/precursors can be used in vitro or fluorescently sorted and transplanted into neonatal neocortex for studying fate determination, or used in therapeutic applications.
