Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream CharacterizationShukun Chen *1, Amin El-Heliebi *1, Julia Schmid 1, Karl Kashofer 2, Zbigniew T. Czyż 3, Bernhard Michael Polzer 3, Klaus Pantel 4, Thomas Kroneis 1,5, Peter Sedlmayr 1
1Institute of Cell Biology, Histology and Embryology, Medical University of Graz, 2Institute of Pathology, Medical University of Graz, 3Fraunhofer Institute for Toxicology and Experimental Medicine ITEM, 4Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, 5Sahlgrenska Cancer Center, University of Gothenburg
This protocol is to recover and prepare rare target cells from a mixture with non-target background cells for molecular genetic characterization at the single-cell level. DNA quality is equal to non-treated single cells and allows for single-cell application (both screening based and targeted analysis).
