Taipei Medical University and National Health Research

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

Chun Austin Changou^1,2,3, Reni Ajoy^4,5, Szu-Yi Chou^4,5,6

¹The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, ²Integrated Laboratory, Center of Translational Medicine, Taipei Medical University, ³Core Facility, Taipei Medical University, ⁴The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, ⁵Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, ⁶TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology, Taipei Medical University

This protocol describes a technique for observation of real-time Green Fluorescence Protein (GFP) tagged Glucose Transporter 4 (GLUT4) protein trafficking upon insulin stimulation and characterization of the biological role of CCR5 in the insulin–GLUT4 signaling pathway with Deconvolution Microscopy.

JoVE Core

Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain

Reni Ajoy^1,2, Szu-Yi Chou^1,2,3

¹The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University and National Health Research, ²Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, ³TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology, Taipei Medical University

This protocol studies the role of chemokine (C-C motif) ligand 5 (CCL5) in the hypothalamus by delivering an antagonist, ^MetCCL5, into the mouse brain using a micro-osmotic pump brain infusion system. This transient inhibition of CCL5 activity interrupted hypothalamic insulin signaling, leading to glucose intolerance and peripheral systemic insulin sensitivity.