Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou

1 ARTICLES PUBLISHED IN JoVE

Chemistry

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

Li Xie¹, Liqin Chen³, Pan Gu⁴, Lanlan Wei¹, Xuejun Kang²

¹School of Public Health of Southeast University, Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, ²Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering, Southeast University, ³School of Public Health, Tianjin Medical University, ⁴British Columbia Academy, Nanjing Foreign Language School

We present a convenient solid-phase extraction coupled to high-pressure liquid chromatography (HPLC) with electrochemical detection (ECD) for simultaneous determination of three monoamine neurotransmitters and two of their metabolites in infants' urine. We also identify the metabolite MHPG as a potential biomarker for the early diagnosis of brain damage for infants.