Universiti Putra Malaysia

3 ARTICLES PUBLISHED IN JoVE

Bioengineering

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Noordiana Nordin¹, Nor Azah Yusof^2,3, Son Radu¹, Roozbeh Hushiarian⁴

¹Laboratory of Food Safety and Food Integrity, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, ²Laboratory of Functional Device, Institute of Advanced Technology, Universiti Putra Malaysia, ³Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, ⁴La Trobe Institute for Molecular Science, La Trobe University

A protocol for the development of an electrochemical DNA biosensor comprising a polylactic acid-stabilized, gold nanoparticles-modified, screen-printed carbon electrode to detect Vibrio parahaemolyticus is presented.

Bioengineering

Human Pluripotent Stem Cell Culture on Polyvinyl Alcohol-Co-Itaconic Acid Hydrogels with Varying Stiffness Under Xeno-Free Conditions

Tzu-Cheng Sung^*1, Hsing-Fen Li^*1, Akon Higuchi^1,2, Qing-Dong Ling^3,4, Jia-Sin Yang¹, Yeh-Chia Tseng¹, Chih-Hsien Pan Pan¹, Abdullah A. Alarfaj², Murugan A. Munusamy², Suresh Kumar⁵, Shih-Tien Hsu⁶, Kadarkarai Murugan^7,8

¹Department of Chemical and Materials Engineering, National Central University, ²Department of Botany and Microbiology, King Saud University, ³Cathay Medical Research Institute, Cathay General Hospital, ⁴Graduate Institute of Systems Biology and Bioinformatics, National Central University, ⁵Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia, ⁶Department of Internal Medicine, Taiwan Landseed Hospital, ⁷Department of Zoology, Bharathiar University, ⁸Thiruvalluvar University

A protocol is presented to prepare polyvinyl alcohol-co-itaconic acid hydrogels with varying stiffness, which were grafted with and without oligopeptides, to investigate the effect of the stiffness of biomaterials on the differentiation and proliferation of stem cells. The stiffness of the hydrogels was controlled by the crosslinking time.

Cancer Research

Time-Lapse 2D Imaging of Phagocytic Activity in M1 Macrophage-4T1 Mouse Mammary Carcinoma Cells in Co-cultures

Noorzaileen Eileena Zaidi¹, Nur Aima Hafiza Shazali², Adam Leow Thean Chor¹, Mohd Azuraidi Osman¹, Kamariah Ibrahim³, Marilyn Jaoi-Edward⁴, Nik Mohd Afizan Nik Abd Rahman^1,2

¹Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, ²Institute of Tropical Forestry and Forestry Products (INTROP), Universiti Putra Malaysia, ³Department of Biomedical Science, Faculty of Medicine, University of Malaya, ⁴Agro-Biotechnology Institute (ABI), National Institute of Biotechnology Malaysia (NIBM)

Macrophage phagocytic activity against cancer cells, specifically 4T1 mouse mammary carcinoma cells, was imaged in this study. The live cell coculture model was established and observed using a combination of fluorescent and differential interference contrast microscopy. This assessment was imaged using imaging software to develop multipoint time-lapse video.