Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus TherapyKristina J. Allan 1,2, Douglas J. Mahoney 1,4, Stephen D. Baird 1, Charles A. Lefebvre 1, David F. Stojdl 1,2,3
1Children's Hospital of Eastern Ontario (CHEO) Research Institute, 2Department of Biology, Microbiology and Immunology, University of Ottawa, 3Department of Pediatrics, University of Ottawa, 4Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary
Here we describe a protocol for employing high-throughput RNAi screening to uncover host targets that can be manipulated to enhance oncolytic virus therapy, specifically rhabodvirus and vaccinia virus therapy, but it can be readily adapted to other oncolytic virus platforms or for discovering host genes that modulate virus replication generally.
