Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico

4 ARTICLES PUBLISHED IN JoVE

Biology

A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants

Katerina Tsilingiri¹, Angelica Sonzogni², Flavio Caprioli³, Maria Rescigno¹

¹Department of Experimental Oncology, European Institute of Oncology, ²Department of Pathology and Laboratory Medicine, European Institute of Oncology, ³U.O. Gastroenterologia 2, IRCCS Ca' Granda, Ospedale Policlinico di Milano

We introduce a novel method for the maintenance of human intestinal mucosa in culture and monitoring of the response to various types of stimuli over at least 24 hrs. With our method, the polarity of the tissue is maintained, allowing for a physiological stimulation via the apical route.

The Efficacy of a Porcine Collagen Matrix in Keratinized Tissue Augmentation: A 6-Month Follow-up Study

Carlo Maiorana¹, Luca Pivetti¹, Fabrizio Signorino¹, Giovanni Battista Grossi¹, Mario Beretta¹, Alan Scott Herford²

¹Implant Center for Edentulism and Jawbone Atrophies, Maxillofacial Surgery and Odontostomatology Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, ²Oral and Maxillofacial Surgery Department, Loma Linda University

The aim of this study was to evaluate the long-term stability of the results achieved in peri implant tissue grafting by means of a collagen matrix.

Immunology and Infection

Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses

Fulvia Milena Cribiù^*1, Claudia Burrello^*2, Roberta Tacchi¹, Francesca Boggio¹, Dario Ricca¹, Flavio Caprioli^3,4, Stefano Ferrero¹, Federica Facciotti²

¹Pathology Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, ²Department of Experimental Oncology, European Institute of Oncology, ³Gastroenterology and Endoscopy Unit, Fondazione IRCCS Cà Granda, Ospedale Maggiore Policlinico, ⁴Department of Pathophysiology and Transplantation, Università degli Studi di Milano

Lack of standardization for murine tissue processing reduces the quality of murine histopathological analysis as compared to human specimens. Here, we present a protocol to perform histopathological examination of murine inflamed and uninflamed colonic tissues to show the feasibility of robotic systems routinely used for processing and embedding human samples.

Medicine

Integrating Augmented Reality Tools in Breast Cancer Related Lymphedema Prognostication and Diagnosis

Marco Invernizzi¹, Letterio Runza², Alessandro De Sire¹, Lorenzo Lippi¹, Concetta Blundo³, Donatella Gambini⁴, Renzo Boldorini^5,6, Stefano Ferrero^2,7, Nicola Fusco^2,7

¹Physical and Rehabilitative Medicine, Department of Health Sciences, University of Eastern Piedmont "A. Avogadro", ²Division of Pathology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, ³Division of Breast Surgery, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, ⁴Division of Medical Oncology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, ⁵Division of Pathology, Maggiore della Carità Hospital, ⁶Department of Health Sciences, University of Eastern Piedmont "A. Avogadro", ⁷Department of Biomedical, Surgical, and Dental Sciences, University of Milan

Breast cancer related lymphedema is frequent in breast cancer survivors, but there are not widely employed guidelines for its diagnosis and quantification. Here, we introduce a reliable and cost-effective protocol to define, quantify, and compare upper limb volume in breast cancer patients.