RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNAManon Torres 1, Denis Becquet 1, Séverine Guillen 1, Bénédicte Boyer 1, Mathias Moreno 1, Marie-Pierre Blanchard 2, Jean-Louis Franc 1, Anne-Marie François-Bellan 1
1CNRS, CRN2M-UMR7286, Faculté de Médecine Nord, Aix-Marseille Université, 2Plate-forme d'imagerie MRI, UMS3426, CNRS 141, Institut de Génétique Humaine
This RNA pull-down method allows identifying the RNA targets of a long non-coding RNA (lncRNA). Based on the hybridization of home-made, designed anti-sense DNA oligonucleotide probes specific to this lncRNA in an appropriately fixed tissue or cell line, it efficiently allows the capture of all RNA targets of the lncRNA.
