University Medical Centre Göttingen
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins
Donatus Riemann 1, Andoniya Petkova 1, Thomas Dresbach 1, Rebecca Wallrafen 1
1Institute for Anatomy and Embryology, University Medical Centre Göttingen
We describe an optical assay for synaptic vesicle (SV) recycling in cultured neurons. Combining this protocol with double transfection to express a presynaptic marker and protein of interest allows us to locate presynaptic sites, their synaptic vesicle recycling capacity, and determine the role of the protein of interest.
Neuroscience
Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence
Rebecca Wallrafen 1, Thomas Dresbach 1, Julio S. Viotti 1
1Institute of Anatomy and Embryology, University Medical Center Göttingen
Here, we describe a quantitative approach to determining the distribution of a synaptic protein relative to a marker protein using immunofluorescence staining, confocal microscopy, and computer-based analysis.
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