Reed College
2 ARTICLES PUBLISHED IN JoVE
Developmental Biology
A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells
Kimberly A. Peters 1, Elizabeth Detmar 1, Liz Sepulveda 2, Corrina Del Valle 2, Ruth Valsquier 2, Anna Ritz 2, Stephen L. Rogers 1,3,4, Derek A. Applewhite 2
1Department of Biology, The University of North Carolina at Chapel Hill, 2Department of Biology, Reed College, 3Carolina Center for Genome Sciences, The University of North Carolina at Chapel Hill, 4Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill
Here we describe a contractility assay using Drosophila S2R+ cells. The application of an exogenous ligand, folded gastrulation (Fog), leads to the activation of the Fog signaling pathway and cellular contractility. This assay can be used to investigate the regulation of cellular contractility proteins in the Fog signaling pathway.
Developmental Biology
Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System
Olivia L. Hagen 1, Yehyun Kim 1, Elaine Kushkowski 1,2, Hannah Rouse 3, Kara L. Cerveny 1
1Department of Biology, Reed College, 2Committee on Development, Regeneration, and Stem Cell Biology, University of Chicago, 3Department of Cell and Developmental Biology, University College London
The article explains how to surgically remove eyes from living zebrafish larvae as the first step toward investigating how retinal input influences optic tectum growth and development. In addition, the article provides information about larval anesthetization, fixation, and brain dissection, followed by immunohistochemistry and confocal imaging.
