University of Maryland School of Medicine, University of Maryland Greenebaum Comprehensive Cancer Center
2 ARTICLES PUBLISHED IN JoVE
Biology
Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
Karthika Natarajan 1,2, Yi Xie 1,3, Takeo Nakanishi 4, Rebecca S. Moreci 5,6, Pancharatnam Jeyasuria 7, Arif Hussain 1,3,8,9, Douglas D. Ross 1,3,8,9,10,11
1Greenebaum Cancer Center, University of Maryland School of Medicine, 2Pharmaceutical Sciences, University of Maryland School of Pharmacy, 3Baltimore VA Medical Center, 4Membrane Transport and Biopharmaceutics, School of Pharmaceutical Sciences, Kanazawa University, 5Obstetrics, Gynecology and Reproductive Science, University of Pittsburgh, 6Magee Women's Research Institute, 7Obstetrics, Gynecology, Perinatal Research Branch (NICHD), Wayne State University School of Medicine, 8Medicine, University of Maryland School of Medicine, 9Pathology, University of Maryland School of Medicine, 10Pharmacology, University of Maryland School of Medicine, 11Experimental Therapeutics, University of Maryland School of Medicine
With the murine ABC transporter Bcrp1 (Abcg2) as an example, in-silico protocols are presented to detect alternative promoter usage in genes expressed in mouse tissues, and to evaluate the functionality of the alternative promoters identified using reporter assays.
Medicine
Proton Therapy Delivery and Its Clinical Application in Select Solid Tumor Malignancies
Adeel Kaiser 1, John G. Eley 1, Nasarachi E. Onyeuku 1, Stephanie R. Rice 1, Carleen C. Wright 2, Nathan E. McGovern 2, Megan Sank 2, Mingyao Zhu 1, Zeljko Vujaskovic 1, Charles B. Simone 2nd 1, Arif Hussain 3
1Department of Radiation Oncology, University of Maryland School of Medicine, 2Department of Radiation Oncology, University of Maryland Medical Center, 3Department of Medicine, University of Maryland School of Medicine, University of Maryland Greenebaum Comprehensive Cancer Center
The fundamentals of radiation planning and delivery for proton therapy using prostate cancer as a model are presented. The application of these principles to other selected disease sites highlights how proton radiotherapy may enhance clinical outcomes for cancer patients.
