Canadian National Transplant Research Program
2 ARTICLES PUBLISHED IN JoVE
Medicine
Normothermic Ex Situ Heart Perfusion in Working Mode: Assessment of Cardiac Function and Metabolism
Sanaz Hatami 1, Christopher W. White 1, Martin Ondrus 1, Xiao Qi 1, Max Buchko 1, Sayed Himmat 1, Lily Lin 2, Katie Cameron 3, David Nobes 4, Hyun-Joong Chung 5, Jayan Nagendran 1,6, Darren H. Freed 1,3,6,7
1Department of Surgery, Faculty of Medicine, University of Alberta, 2Department of Pediatrics, Faculty of Medicine, University of Alberta, 3Department of Biomedical Engineering, Faculty of Medicine, University of Alberta, 4Department of Mechanical Engineering, Faculty of Engineering, University of Alberta, 5Department of Chemical and Materials Engineering, Faculty of Engineering, University of Alberta, 6Canadian National Transplant Research Program, 7Department of Physiology, Faculty of Medicine, University of Alberta
Normothermic ex situ heart perfusion (ESHP), preserves the heart in a beating, semi-physiologic state. When performed in a working mode, ESHP provides the opportunity to perform sophisticated assessments of donor heart function and organ viability. Here, we describe our method for myocardial performance evaluation during ESHP.
Medicine
Normothermic Negative Pressure Ventilation Ex Situ Lung Perfusion: Evaluation of Lung Function and Metabolism
Keir A. Forgie 1,2, Nicholas Fialka 3, Max Buchko 1,2, Sayed Himmat 4, Sanaz Hatami 4, Xiao Qi 4, Xiuhua Wang 4, Katie-Marie Buswell 5, Ryan Edgar 5, Darren H. Freed 1,2,6,7, Jayan Nagendran 1,2,6,7
1Division of Cardiac Surgery, Department of Surgery, Faculty of Medicine, University of Alberta, 2Mazankowski Alberta Heart Institute, 3Faculty of Medicine and Dentistry, University of Alberta, 4Department of Surgery, Faculty of Medicine, University of Alberta, 5Ray Rajotte Surgical-Medical Research Institute (SMRI), University of Alberta, 6Alberta Transplant Institute, 7Canadian Donation and Transplantation Research Program
This paper describes a porcine model of negative pressure ventilation ex situ lung perfusion, including procurement, attachment, and management on the custom-made platform. Focus is made on anesthetic and surgical techniques, as well as troubleshooting.
