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Niguarda Hospital

2 ARTICLES PUBLISHED IN JoVE

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Medicine

Endotoxin Activity Assay for the Detection of Whole Blood Endotoxemia in Critically Ill Patients
Riccardo Pinciroli 1,2, Simone Checchi 2, Maurizio Bottiroli 1, Gianpaola Monti 1, Giampaolo Casella 1, Roberto Fumagalli 1,2
1Department of Anesthesia and Critical Care, Niguarda Hospital, 2University of Milan-Bicocca Medical School

We hereby present a protocol to measure at the bedside the endotoxin activity of human whole blood samples. The Endotoxin Activity assay is a simple test to perform and may be a useful biomarker in critically ill patients with sepsis.

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Medicine

A Novel Inhalation Mask System to Deliver High Concentrations of Nitric Oxide Gas in Spontaneously Breathing Subjects
Riccardo Pinciroli 1, Lisa Traeger 1, Anna Fischbach 1, Stefano Gianni 1, Caio Cesar Araujo Morais 1, Bijan Safaee Fakhr 1, Raffaele Di Fenza 1, Dianna Robinson 3, Ryan Carroll 2, Warren M. Zapol 1, Lorenzo Berra 1
1Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital and Harvard Medical School, 2Division of Pediatric Critical Care Medicine, Department of Pediatrics, Massachusetts General Hospital and Harvard Medical School, 3Respiratory Care, Massachusetts General Hospital

This simple and highly adaptable system device for the inhalation of high-concentration nitric oxide (NO) gas does not require mechanical ventilators, positive pressure, or high gas flows. Standard medical consumables and a snug-fitting mask are used to safely deliver NO gas to spontaneously breathing subjects.

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