Johns Hopkins School of Public Health

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure

Jenny L. Carey¹, Olivia H. Cox¹, Fayaz Seifuddin¹, Leonard Marque¹, Kellie L.K. Tamashiro¹, Peter P. Zandi^1,3, Gary S. Wand^1,2, Richard S. Lee¹

¹Departments of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, ²Department of Medicine, Johns Hopkins School of Medicine, ³Department of Mental Health, Johns Hopkins School of Public Health

Here, we describe the protocol and implementation of Methyl-Seq, an epigenomic platform, using a rat model to identify epigenetic changes associated with chronic stress exposure. Results demonstrate that the rat Methyl-Seq platform is capable of detecting methylation differences that arise from stress exposure in rats.