Automated Behavioral Analysis of Large C. elegans Populations Using a Wide Field-of-view Tracking PlatformMichele Perni 1, Sam Casford 1, Francesco A. Aprile 1, Ellen A. Nollen 2, Tuomas P.J. Knowles 1, Michele Vendruscolo 1, Christopher M. Dobson 1
1Centre for Misfolding Diseases, Department of Chemistry, University of Cambridge, 2European Research Institute for the Biology of Aging, University Medical Centre Groningen
We describe protocols for using the wide field-of-view nematode tracking platform (WF-NTP), which enables high-throughput phenotypic characterization of large populations of Caenorhabditis elegans. These protocols can be used to characterize subtle behavioral changes in mutant strains or in response to pharmacological treatment in a highly scalable fashion.
