VIB-KU Leuven Center for Brain and Disease Research

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors

Shelly Fripont^*1,2, Catherine Marneffe^*1,2, Marika Marino^*1,2, Melvin Y. Rincon^1,2, Matthew G. Holt^1,2,3

¹VIB-KU Leuven Center for Brain and Disease Research, ²Department of Neuroscience, KU Leuven, ³Leuven Brain Institute

Here, we describe an efficient and reproducible strategy to produce, titer, and quality-control batches of adeno-associated virus vectors. It allows the user to obtain a vector preparation with high-titer (≥1 x 10¹³ vector genomes/mL) and a high purity, ready for in vitro or in vivo use.