University of Illinois Cancer Center
2 ARTICLES PUBLISHED IN JoVE
Medicine
Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
Giovanni Lugli 1, Yachana Kataria 1, Zachary Richards 1, Peter Gann 1, Xiaofeng Zhou 2, Larisa Nonn 1
1Department of Pathology, University of Illinois at Chicago, 2Department of Periodontics, University of Illinois at Chicago
The goal of this protocol is to use laser-capture micro-dissection as an effective method to isolate pure populations of cell types from heterogeneous prostate tissues for downstream RNA analysis.
Bioengineering
Handling and Assessment of Human Primary Prostate Organoid Culture
Tara McCray *1, Zachary Richards *1, Joseph Marsili 1, Gail S. Prins 1,2,3, Larisa Nonn 1,3
1Department of Pathology, University of Illinois at Chicago, 2Departments of Urology, Physiology, and Biophysics, University of Illinois at Chicago, 3University of Illinois Cancer Center
Here, we present a protocol to guide human primary prostate organoid handling then suggest endpoints to assess phenotype. Seeding, culture maintenance, recovery from matrix gel, morphologic quantification, embedding and sectioning, FFPE sectioning, whole-mount staining, and application of commercial assays are described.
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