Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass SpectrometryJerlisa Ann C. Arizala 1,2, Pritsana Chomchan 1, Haitang Li 1, Roger Moore 3, Helen Ge 3, Dominique L. Ouellet 1, John J. Rossi 1,2
1Molecular and Cellular Biology Department, Beckman Research Institute at the City of Hope, 2Irell & Manella Graduate School of Biological Sciences, 3Department of Molecular Immunology, Beckman Research Institute and the City of Hope
Here we describe Rev immunoprecipitation in the presence of HIV-1 replication for mass spectrometry. The methods described can be used for the identification of nucleolar factors involved in the HIV-1 infectious cycle and are applicable to other disease models for the characterization of understudied pathways.
