Processing of Bronchoalveolar Lavage Fluid and Matched Blood for Alveolar Macrophage and CD4+ T-cell Immunophenotyping and HIV Reservoir AssessmentSyim Salahuddin *1,2, Elaine Thomson *1,2,3, Oussama Méziane 1,2, Omar Farnos 2, Amélie Pagliuzza 5, Nicolas Chomont 5,6, Ron Olivenstein 1, Cecilia Costiniuk 1,3,4, Mohammad-Ali Jenabian 2,3,6
1Research Institute McGill University Health Centre, 2Department of Biological Sciences, Université de Québec à Montréal, 3Department of Microbiology & Immunology, McGill University, 4Department of Medicine, Division of Infectious Diseases, McGill University, 5Centre de Recherche du Centre Hospitalier de l'Université de Montréal, 6Département de microbiologie, infectiologie et immunologie, Université de Montréal
We describe a method for processing bronchoalveolar lavage fluid and matched peripheral blood from chronically HIV-infected individuals on antiretroviral therapy to assess pulmonary HIV reservoirs. These methods result in the acquisition of highly pure CD4 T cells and alveolar macrophages that may subsequently be used for immunophenotyping and HIV DNA/RNA quantifications by ultrasensitive polymerase chain reaction.
