Showa University School of Medicine

4 ARTICLES PUBLISHED IN JoVE

Cancer Research

Establishing Dual Resistance to EGFR-TKI and MET-TKI in Lung Adenocarcinoma Cells In Vitro with a 2-step Dose-escalation Procedure

Toshimitsu Yamaoka¹, Motoi Ohba¹, Satoru Arata^1,2, Tohru Ohmori¹

¹Institute of Molecular Oncology, Showa University, ²Center for Biotechnology, Showa University

An in vitro method for establishing dual resistance to an EGFR-TKI and a MET-TKI in cancer cells is described. This method is useful for developing treatments for patients with EGFR-mutations, who exhibit disease progression despite EGFR-TKI treatment with MET-amplification. It can also be modified for inhibitors targeting other molecules.

Cancer Research

Establishment and Characterization of Three Afatinib-resistant Lung Adenocarcinoma PC-9 Cell Lines Developed with Increasing Doses of Afatinib

Toshimitsu Yamaoka¹, Motoi Ohba¹, Yuki Matsunaga², Junji Tsurutani¹, Tohru Ohmori³

¹Advanced Cancer Translational Research Institute, Showa University, ²Division of Breast Surgical Oncology, Department of Surgery, Showa University School of Medicine, ³Division of Allergology and Respiratory Medicine, Department of Medicine, Showa University School of Medicine

A method for establishing afatinib-resistance cell lines from lung adenocarcinoma PC-9 cells was developed, and resistant cells were characterized. The resistant cells can be used to investigate epidermal growth factor receptor tyrosine kinase inhibitor-resistance mechanisms, applicable for patients with non-small cell lung cancer.

JoVE Journal

Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique

Takashi Obama^*1, Takuro Miyazaki^*2, Toshihiro Aiuchi¹, Akira Miyazaki², Hiroyuki Itabe¹

¹Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, ²Department of Biochemistry, Showa University School of Medicine

Here, we present a protocol for an on-membrane digestion technique for the preparation of samples for mass spectrometry. This technique facilitates the convenient analysis of protein–protein interactions.

Medicine

Minimally Invasive Treatment for Thoracolumbar Burst Fracture Using Sagittal Alignment Screws and A Trauma Reduction Device

Joji Ogawa^*1,2, Yusuke Dodo^*2, Yoshihisa Komuro^2,3, Chikara Hayakawa², Ichiro Okano^2,3

¹Department of Orthopaedic Surgery, Tokyo Metropolitan Hiroo Hospital, ²Department of Orthopaedic Surgery, Showa University School of Medicine, ³Department of Orthopaedic Surgery, Ohta-Nishinouchi Hospital

The sagittal adjusting screw (SAS) system has been widely used for thoracolumbar (TL) spinal trauma. Percutaneous options and specialized trauma reduction devices are also available for the SAS system. We describe a technique for reducing TL burst fractures using an SAS system and a newly introduced trauma reduction device.