Singapore-MIT Alliance for Research and Technology, CREATE
3 ARTICLES PUBLISHED IN JoVE
Bioengineering
Studying Cell Rolling Trajectories on Asymmetric Receptor Patterns
Chia-Hua Lee 1, Suman Bose 2, Krystyn J. Van Vliet 1, Jeffrey M. Karp 3, Rohit Karnik 2
1Department of Materials Science and Engineering, MIT - Massachusetts Institute of Technology, 2Department of Mechanical Engineering, MIT - Massachusetts Institute of Technology, 3HST Center for Biomedical Engineering and Harvard Stem Cell Institute, Brigham and Women's Hospital and Harvard Medical School
We describe a protocol to observe and analyze cell rolling trajectories on asymmetric receptor-patterned substrates. The resulting data are useful for engineering of receptor-patterned substrates for label-free cell separation and analysis.
Neuroscience
Characterizing Multiscale Mechanical Properties of Brain Tissue Using Atomic Force Microscopy, Impact Indentation, and Rheometry
Elizabeth Peruski Canovic 1, Bo Qing 2, Aleksandar S. Mijailovic 3, Anna Jagielska 2, Matthew J. Whitfield 2, Elyza Kelly 4, Daria Turner 4, Mustafa Sahin 4, Krystyn J. Van Vliet 1,2
1Department of Materials Science and Engineering, Massachusetts Institute of Technology, 2Department of Biological Engineering, Massachusetts Institute of Technology, 3Department of Mechanical Engineering, Massachusetts Institute of Technology, 4Department of Neurology, The F.M. Kirby Neurobiology Center, Boston Children’s Hospital, Harvard Medical School
We present a set of techniques to characterize the viscoelastic mechanical properties of brain at the micro-, meso-, and macro-scales.
Bioengineering
High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain
Ekta Makhija 1, Anna Jagielska 1,2, Krystyn J. Van Vliet 1,2,3
1BioSystems and Micromechanics Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, CREATE, 2Department of Material Science and Engineering, Massachusetts Institute of Technology, 3Department of Biological Engineering, Massachusetts Institute of Technology
Using a previously designed device to apply mechanical strain to adherent cells, this paper describes a redesigned substratum geometry and a customized apparatus for high-resolution single-cell imaging of strained cells with a 100x oil immersion objective.
