School of Engineering École Polytechnique Fédérale de Lausanne (EPFL)
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
Ardjan J. van der Linden 1, Maaruthy Yelleswarapu 2, Pascal A. Pieters 1, Zoe Swank 3, Wilhelm T. S. Huck 2, Sebastian J. Maerkl 3, Tom F. A. de Greef 1,2
1Institute for Complex Molecular Systems, Department of Biomedical Engineering, Computational Biology Group, Eindhoven University of Technology, 2Institute for Molecules and Materials, Radboud University, 3Institute of Bioengineering, School of Engineering École Polytechnique Fédérale de Lausanne (EPFL)
The fabrication process of a PDMS-based, multilayer, microfluidic device that allows in vitro transcription and translation (IVTT) reactions to be performed over prolonged periods is described. Furthermore, a comprehensive overview of the hardware and software required to automate and maintain these reactions for prolonged durations is provided.
Bioengineering
OnePot PURE Cell-Free System
Laura Grasemann *1, Barbora Lavickova *1, M. Carolina Elizondo-Cantú 1, Sebastian J. Maerkl 1
1Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne
We present a fast and cost-effective method to produce the recombinant PURE cell-free TX-TL system using standard laboratory equipment.
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