Institute of Molecular Genetics of the Czech Academy of Sciences

4 ARTICLES PUBLISHED IN JoVE

Neuroscience

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

¹Laboratory of RNA Biology, Institute of Molecular Genetics of the Czech Academy of Sciences, ²Department of Genetics and Microbiology, Faculty of Science, Charles University

Biogenesis of spliceosomal snRNAs is a complex process involving various cellular compartments. Here, we employed microinjection of fluorescently labelled snRNAs in order to monitor their transport inside the cell.

Biology

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

Katerina Korelova¹, Marketa Jirouskova¹, Lenka Sarnova¹, Martin Gregor¹

¹Laboratory of Integrative Biology, Institute of Molecular Genetics of the Czech Academy of Sciences

Presented here is a protocol for the isolation of mouse hepatocytes from adult mouse livers using a modified collagenase perfusion technique. Also described is the long-term culture of hepatocytes in a 3D collagen sandwich setting as well as immunolabeling of the cytoskeletal components to study bile canalicular formation and its response to treatment.

Biology

Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy

Srikant Ojha¹, Jan Pribyl², Simon Klimovic^2,3, Daniel Hadraba⁴, Marketa Jirouskova¹, Martin Gregor¹

¹Laboratory of Integrative Biology, Institute of Molecular Genetics of the Czech Academy of Sciences, ²CEITEC MU, Masaryk University, ³Department of Biochemistry, Faculty of Science, Masaryk University, ⁴Laboratory of Biomathematics, Czech-BioImaging IPHYS, Institute of Physiology of the Czech Academy of Sciences

We present a protocol to measure the elastic moduli of collagen-rich areas in normal and diseased liver using atomic force microscopy. The simultaneous use of polarization microscopy provides high spatial precision for localizing collagen-rich areas in the liver sections.

Behavior

Behavioral Characterization of an Angelman Syndrome Mouse Model

Agnieszka Kubik-Zahorodna¹, Jan Prochazka^1,2, Radislav Sedlacek^1,2

¹Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, ²Laboratory of Transgenic Models of Diseases, Institute of Molecular Genetics of the Czech Academy of Sciences

This manuscript presents a set of highly reproducible behavioral tests to validate an Angelman syndrome mouse model.