Center for iPS cell research and application (CiRA)

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

Ryo Ohta^*1, Ryohichi Sugimura^*1, Akira Niwa¹, Megumu K. Saito¹

¹Center for iPS cell research and application (CiRA)

Here, we present a protocol to precisely form hemogenic endothelium from human pluripotent stem cells in a feeder- and xeno-free condition. This method will have broad applications in disease modeling and screening for therapeutic compounds.

Developmental Biology

In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells

Chuang-Yu Lin¹, Michiko Yoshida^1,2, Li-Tzu Li³, Megumu K. Saito¹

¹Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, ²Department of Pediatrics, Kyoto Prefectural University of Medicine, ³Graduate Institute of Clinical Medicine, Taipei Medical University

Here we provide a protocol to generate in vitro NMJs from human induced pluripotent stem cells (iPSCs). This method can induce NMJs with mature morphology and function in 1 month in a single well. The resulting NMJs could potentially be used to model related diseases, to study pathological mechanisms or to screen drug compounds for therapy.