Sackler School of Graduate Biomedical Sciences

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers

Alyssa C. Fasciano¹, Sarah E. Blutt², Mary K. Estes², Joan Mecsas³

¹Program in Immunology, Sackler School of Graduate Biomedical Sciences, ²Department of Molecular Virology and Microbiology, Baylor College of Medicine, ³Department of Molecular Biology and Microbiology, Tufts University

This protocol describes how to induce the differentiation of M cells in human stem cell-derived ileal monolayers and methods to assess their development.